Sempere Laboratory

Selected Publications

For a full list of Dr. Sempere’s publications, please visit PubMed here.


Fromm B, Tyler BJ, Morten J, Peck LE, Tarver JE, King BL, Newcomb JM, Sempere LF, Flatmark K, Hovig E, Peterson KJ. In press. A uniform system for the annotation of human microRNA genes and the evolution of the human microRNAome. Annu Rev Gen. 49(1) doi:10.1146/annurev-genet-120213-092023.

Ensink E, Sinha J, Sinha A, Tang H, Calderone HM, Hostetter G, Winter J, Cherba D, Brand RE, Allen PJ, Sempere LF, Haab BB. 2015. Segment and fit thresholding: A new method for image analysis applied to microarray and immunofluorescence data. Anal Chem. 87(19):9715–9721. PubMed

Machiela E, Popkie A, Sempere LF. 2015. Individual noncoding RNA variations: Their role in shaping and maintaining the epigenetic landscape In: Trygve Tollefsbol, ed.  Waltham, Massachusetts: Academic Press 2015. Chapter 4 pages:84-114. ISBN-13: 978-0124201354

Danilova O, Paiva C, Kaur P, Kamal A, Sempere LF, Danilov A. 2015. microRNA-21 is differentially expressed in the lymphoid tissue and modulated by stromal signaling in chronic lymphocytic leukemia (CLL). British J Haematol. doi: 10.1111/bjh.13282.    PubMed    Article

Andrew AS, Marsit CJ, Schned AR, Seigne JD, Kelsey KT, Cowper R, Moore JH, Perreard J, Karagas MR, Sempere LF. 2015. Expression of tumor suppressive microRNA-34a is associated with a reduced risk of bladder cancer recurrence. Int J Cancer. doi: 10.1002/ijc.29413.     PubMed    Article

Graveel CR, Calderone HM, Westerhuis J, Winn ME, Sempere LF. 2015. Critical analysis of the potential for microRNA biomarkers in breast cancer management. Breast Cancer (Dove Med Press). 7:59–79.     Article      Video abstract


MacKenzie TA, Schwartz G, Calderone HM, Graveel CR, Winn ME, Hostetter G, Wells WA, Sempere LF. Stromal expression of miR-21 identifies high-risk group in triple-negative breast cancer. Am J Pathol. 184(12): 3217–3225. PubMed

Sempere LF. Fully automated fluorescence-based 4-color multiplex assay for co-detection of microRNA and protein biomarkers in clinical tissue specimens. 2014. In In Situ Hybridization Protocols, Boye Schnack Nielsen, ed.  Methods in Molecular Medicine series, Berlin: Springer. PubMed

Sempere LF. 2014. Tissue slide-based microRNA characterization of tumors: how detailed could diagnosis become for cancer medicine?  Expert Rev Mol Diagn. 14(7):853-69. PubMed

Lines JL, Sempere LF, Broughton T, Wang L, Noelle R. 2014. VISTA is a novel broad-spectrum negative checkpoint regulator for cancer immunotherapy. Cancer Immunol Res. 2(6):510-7. PubMed

Lines JL, Pantazi ED, Mak J, Sempere LF, Wang L, O’Connell S, Ceeraz S, Suriawinata AA, Yan S, Ernstoff MS, Noelle RJ. 2014. VISTA is an immune checkpoint molecule for human T cells. Cancer Res. 74(7):1924-32. PubMed


Ma T, Galimberti F, Erkmen CP, Memoli V, Chinyengetere F, Sempere LF, Beumer JH, Anyang BN, Nugent W, Johnstone D, Tsongalis GJ, Kurie J, Li H, DiRenzo J, Guo Y, Freemantle SJ, Dragnev KH, Dmitrovsky E. 2013. Comparing histone deacetylase inhibitor responses in genetically engineered mouse lung cancer models and a window of opportunity trial in lung cancer patients. Mol Cancer Ther. 12(8):1545-55. PubMed

Sempere LF*, Korc M. A method for conducting highly sensitive microRNA in situ hybridization and immunohistochemical analysis in pancreatic cancer. In: Gloria H. Su, editor, Pancreatic Cancer: Methods and Protocols, Methods in Molecular Biology, vol. 980. Springer Science+Business Media, LLC, 2013, pp. 43-59. PubMed 
*Corresponding author

Balboni AL, Hutchinson JA, Decastro AJ, Cherukuri P, Liby KT, Sporn MB, Schwartz G, Wells WA, Sempere LF, Yu P, DiRenzo J. 2013. Np63a mediated activation of bone morphogenetic protein signaling governs stem cell activity and plasticity in normal and malignant mammary epithelial cells. Cancer Res. 73(2):1020-30. PubMed


Mercatelli N, Beldiman C, Brouard T, Nairismägi ML, Kratassiouk, G, Largitte LA, Morozova N, Sempere LF, Pritchard LL, Groisman R, Harel-Bellan, A. miR-205 modulates acinar size and morphology of transformed breast epithelial cells. OncomiRs. Volume 1, Pages 2–9, Dec 2012. ISSN

Cubillos-Ruiz JR, Sempere LF, Conejo-Garcia JR. 2012. Good things come in small packages: therapeutic anti-tumor immunity induced by microRNA nanoparticles. Oncoimmunology. 1(6):968-970. PubMed

Sempere LF. 2012. Recent advances in miRNA-based diagnostic applications. Expert Rev Mol Diagn. 12(6):557-9. PubMed

Cubillos-Ruiz JR, Baird JR, Tesone AJ, Rutkowski MR, Scarlett UK, Camposeco-Jacobs AL, Anadon-Arnillas J, Harwood NM, Korc M, Fiering SN, Sempere LF, Conejo-Garcia JR. 2012. Reprogramming tumor-associated dendritic cells in vivo using microRNA mimetics triggers protective immunity against ovarian cancer. Cancer Res. 72(7):1683-93. PubMed

Sempere LF*, Conejo-Garcia JR. Modulation of cancer progression by tumor microenvironmental leukocyte-expressed microRNAs. In: Subhra K. Biswas, editor, Tumor Microenvironment and Myelomonocytic Cells. InTech Open Access Publisher, 2012, pp. 221-254. ISBN 978-953-51-0439-1
*Corresponding author


Sempere LF. 2011. Integrating contextual microRNA and protein signatures for diagnostic and treatment decisions in cancer. Expert Rev Mol Diagn. 11(8):813-27. PubMed

Preis M, Gardner TB, Gordon SR, Pipas MJ, Mackenzie TA, Klein EE, Longnecker DS, Gutmann EJ, Sempere LF*, Korc M. 2011. microRNA-10b expression correlates with response to neoadjuvant therapy and survival in pancreatic ductal adenocarcinoma. Clin Cancer Res. 17(17):5812-21. PubMed
*Corresponding author

Sempere LF*, Kauppinen S. Translational implications of microRNAs in clinical diagnostics and therapeutics. In: Edward A. Dennis and Ralph A. Bradshaw, editors, Intercellular Signaling in Development and Disease. Oxford: Academic Press, 2011 (reprint from Ralph A. Bradshaw and Edward A. Dennis, editors, Handbook of Cell Signaling 2nd edition. Oxford: Academic Press, 2009, pp. 2965-2981). ISBN 978-0-12-382215-4 
*Corresponding author.

Sempere LF*, Gunn J, Korc M. 2011. A novel three-dimensional culture system uncovers growth stimulatory actions by TGF-β in pancreatic cancer cells. Cancer Biol Ther. 12(3). 198-207 PubMed
*Corresponding author

Liu X, Sempere LF, Guo Y, Korc M, Kauppinen S., Freemantle SJ, Dmitrovsky E. 2011. Involvement of microRNAs in lung cancer biology and therapy. Transl Res. 157(4):200-8. PubMed


Sempere LF*, Preis M, Yezefski T, Ouyang H, Suriawinata AA, Silahtaroglu A, Conejo-Garcia JR, Kauppinen S, Wells W, Korc M. 2010. Fluorescence-based codetection with protein markers reveals distinct cellular compartments for altered microRNA expression in solid tumors. Clin Cancer Res. 16, 4246-4255. PubMed 
*Corresponding author

Liu X, Sempere LF, Ouyang H, Memoli VA, Andrew AS, Luo Y, Demidenko E, Korc M, Shi W, Preis M, Dragnev KH, Li H, DiRenzo J, Bak M, Freemantle SJ, Kauppinen S, Dmitrovsky E. 2010. MicroRNA-31 functions as an oncogenic microRNA in mouse and human lung cancer cells by repressing specific tumor suppressors. J Clin Invest. 120(4):1298–1309. PubMed

2009 and earlier

Liu X, Sempere LF, Galimberti F, Freemantle S, Black C, Fiering S, Cole C, Dragnev K, Memoli V, Korc M, Li H, DiRenzo J, Bak M, Kauppinen S, Dmitrovsky E. 2009. Uncovering growth suppressive microRNAs in lung cancer. Clin Cancer Res. 15(4):1177-83. PubMed

Heimberg AM, Sempere LF, Moy VN, Donoghue PC, Peterson KJ. 2008. MicroRNAs and the advent of vertebrate morphological complexity. Proc Natl Acad Sci U S A. 105(8):2946-50. PubMed

Sempere LF, Christensen M, Silahtaroglu A, Bak M, Heath CV, Schwartz G, Wells W, Kauppinen S, Cole CN. 2007. Altered microRNA expression confined to specific epithelial cell subpopulations in breast cancer. Cancer Res. 67(24):11612-20. PubMed
*Corresponding author

Sempere LF, Cole CN, McPeek MA, Peterson KJ. 2006. The phylogenetic distribution of metazoan microRNAs: Insights into evolutionary complexity and constraint. J Exp Zool (Mol Dev Evol). 306B:575-588. PubMed
Featured article on the journal cover page

Sempere LF, Freemantle S, Pitha-Rowe I, Moss E, Dmitrovsky E, Ambros V. 2004. Expression profiling of mammalian microRNAs uncovers a subset of brain-expressed microRNAs with possible roles in murine and human neuronal differentiation. Genome Biol. 5, R13. PubMed

Sempere LF, Sokol NS, Dubrovsky EB, Berger EM, Ambros V. 2003. Temporal regulation of microRNA expression in Drosophila melanogaster mediated by hormonal signals and broad-complex gene activity. Dev Biol. 259(1):9-18. PubMed

Sempere LF, Dubrovsky EB, Dubrovskaya VA, Berger EM, Ambros V. 2002. The expression of the let-7 small regulatory RNA is controlled by ecdysone during metamorphosis in Drosophila melanogasterDev Biol.  244(1):170–179.  PubMed